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Addgene inc plasmid
Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqe9+his+p97/pQE9-His-p97(wt)+(Plasmid+%2314666)/pmc11214410-81-0-7
Average 92 stars, based on 7 article reviews
plasmid - by Bioz Stars, 2026-09
92/100 stars

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Related Articles

Variant Assay:

Article Title: Designing a chemical inhibitor of the AAA protein spastin using active site mutations
Article Snippet: .. Mus musculus VCP vector. pQE9-His-p97 (100% identical to Homo sapiens VCP protein, fulllength, NCBI RefSeq NP_009057.1, Ile206Val variant) was obtained from Addgene (G. Warren, plasmid #14666). ..

Article Title: Designing a chemical inhibitor for the AAA protein spastin using active site mutations.
Article Snippet: .. Mus musculus VCP vector. pQE9-His-p97 (identical to Homo sapiens VCP protein, full length, NCBI RefSeq: NP_009057.1, Ile206Val variant) was obtained from Addgene (G. Warren, plasmid #14666). ..

Plasmid Preparation:

Article Title: Designing a chemical inhibitor of the AAA protein spastin using active site mutations
Article Snippet: .. Mus musculus VCP vector. pQE9-His-p97 (100% identical to Homo sapiens VCP protein, fulllength, NCBI RefSeq NP_009057.1, Ile206Val variant) was obtained from Addgene (G. Warren, plasmid #14666). ..

Article Title: Designing a chemical inhibitor for the AAA protein spastin using active site mutations.
Article Snippet: .. Mus musculus VCP vector. pQE9-His-p97 (identical to Homo sapiens VCP protein, full length, NCBI RefSeq: NP_009057.1, Ile206Val variant) was obtained from Addgene (G. Warren, plasmid #14666). ..

Construct:

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAGCMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) (23–25). ..

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAG-CMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) ( 23 , – 25 ). ..

Generated:

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAGCMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) (23–25). ..

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAG-CMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) ( 23 , – 25 ). ..

Mutagenesis:

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAGCMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) (23–25). ..

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAG-CMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) ( 23 , – 25 ). ..

Ubiquitin Proteomics:

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAGCMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) (23–25). ..

Article Title: The UBX Protein SAKS1 Negatively Regulates Endoplasmic Reticulum-associated Degradation and p97-dependent Degradation
Article Snippet: The vectors pFLAG-CMV-4 (Sigma) and pGEX-6P-1 (GE Biosciences) were used for mammalian expression of FLAG-tagged proteins and the bacterial expression of GST-tagged proteins, respectively. .. All point-mutated constructs were generated using the QuikChange site-directed mutagenesis kit (Stratagene). pQE9-His-p97 (wild type), ubiquitin-G76V-GFP, and pGEX hHR23A were obtained from Addgene (Addgene plasmids 14666, 11941, and 10864) ( 23 , – 25 ). ..



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Image Search Results


Journal: iScience

Article Title: A non-symmetrical p97 conformation initiates a multistep recruitment of Ufd1/Npl4

doi: 10.1016/j.isci.2024.110061

Figure Lengend Snippet:

Article Snippet: Plasmid: pQE9-His-p97(WT) , Meyer et al. , Addgene #14666.

Techniques: Virus, Recombinant, Modification, Western Blot, Bicinchoninic Acid Protein Assay, Structural Proteomics, Mutagenesis, Plasmid Preparation, Software

KEY RESOURCES TABLE

Journal: Cell chemical biology

Article Title: Analyzing resistance to design selective chemical inhibitors for AAA proteins

doi: 10.1016/j.chembiol.2019.06.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: pQE9-His-p97(wt) ( M. musculus VCP/p97) , (Meyer et al. 2000), Addgene #14666 , https://www.addgene.org/14666/.

Techniques: Recombinant, Protease Inhibitor, Software